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Canadian Macromolecular Crystallography Facility

User Guide

The Canadian Macromolecular Crystallography Facility (CMCF) consists of two beamlines, 08ID-1 and 08B1-1, along with supporting infrastructure including a sample preparation room, sample storage room and access to wet lab facilities.

08ID-1

The scientific goal of the 08ID-1 beamline is to operate a protein crystallography beamline suitable for studying small crystals and crystals with large unit cells. The overall design of the beamline contains white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly cryo-cooled first crystal and sagittally focusing second crystal, and vertically focussing mirror (VFM). The beamline is completed with an innovative and very robust endstation with MarMosaic Rayonix MX300 CCD X-ray detector. The beamline controls are based on EPICS and are complemented with a MxDC user interface.

The beamline is equipped with a Röntec XFlash 1001 to perform X-ray spectroscopy for MAD and X-ray absorption near edge structure (XANES) on the same crystals, and X-ray fluorescence (XRF) for metal tracing on protein derivative crystals.

Brief Details

Source	20 mm asymmetric hybrid small-gap in-vacuum undulator (SGU)
Minimum Energy	6.5 keV (1.9 Å)
Maximum Energy	18 keV (0.69 Å)
Flux on Sample @ 12 keV	>10¹² ph/s
Focal Size (FWHM) @ 12 keV (H x V)	150 µm x 50 µm
Techniques	Single crystal X-ray diffraction Multiwavelength Anomalous Dispersion (MAD) XANES on crystals
Crystal-Detector Distance	100-900 mm
Detector Model	Rayonix MX300 CCD X-ray detector
X-ray Sensitive Surface	300 x 300 mm (90,000 mm²)

08B1-1

Designed for complmentary performance to the existing 08ID-1 bealmine, the 08B1-1 beamline will be used for screening crystals and for high-throughput crystallographic analysis of well-ordered crystals. The design incorporates a vertical focussing mirror (VFM), white beam slits (WBS), a double crystal monochromator (DCM) with an indirectly water-cooled first crystal, and a toroidal focusing mirror. The endstation includes a versatile ACCEL MD2 microdiffractometer and MarMosaic mx300 CCD X-ray detector. The beamline controls are operated through the same Blu-Ice style user interface used by 08ID-1. The endstation is equiped with an automounter robot allowing for remote experiments as well as onsite work.

Brief Details

Source	Bending magnet
Minimum Energy	4.0 keV (3.1 Å)
Maximum Energy	18 keV (0.69 Å)
Flux on Sample @ 12 keV	> 10¹¹ ph/s 1 mrad horizontal fan
Focal Size (FWHM) @ 12 keV (H x V)	230 µm x 160 µm
Techniques	Single X-ray diffraction Multiwavelength Anomalous Dispersion (MAD) XANES on crystals
Crystal-Detector Distance	100-500 mm
Detector Model	Rayonix MX300HE CCD X-ray detector
X-ray Sensitive Surface	300 x 300 mm (90,000 mm²)

Additional Details

The source of X-rays on 08ID-1 is a small-gap undulator inserted into the storage ring (and is hence termed an "insertion device"). As its name suggests, the undulator produces a periodic magnetic field that causes the path of the orbiting electrons to undulate, resulting in an emitted X-ray beam of higher spectral brilliance than could be produced by the simpler path taken by the electrons through a bending magnet.

(Image derived from Insertion Devices Primer by Eric Johnson, Advanced Design Consulting USA)

The lower photon flux means that exposure times for crystals on the 08B1-1 beamline will be longer than on the 08ID-1 beamline. The bending magnet of the 08B1-1 beamline also happens to produce maximum flux at about 4.0 keV, and the beamline has been been constructed with these useful lower photon energies in mind. (In particular, 4.0 keV is the point at which the anomalous and dispersive signals from nautrally-occurring sulfur atoms occurs.)

Floor Plan with 08B1-1 and 08ID-1 Highlighted

This floor plan shows the location of the 08ID-1 and 08B1-1 beamlines in relation to the CLS beamline hall.